Restriction Site–Specific Methylation Studies of Imprinted Genes with Quantitative Real-Time PCR
نویسندگان
چکیده
منابع مشابه
Restriction site-specific methylation studies of imprinted genes with quantitative real-time PCR.
BACKGROUND Epigenetic studies, such as the measurement of DNA methylation, are important in the investigation of syndromes influenced by imprinted genes. Quick and accurate quantification of methylation at such genes can be of appreciable diagnostic aid. METHODS We first digested genomic DNA with methylation-sensitive restriction enzymes and used DNA without digestion as a control and nonmeth...
متن کاملMethylation analysis by restriction endonuclease digestion and real-time PCR.
DNA methylation is a highly characterized epigenetic modification of the human genome that is critical for normal cell processes, including tissue regulation, genetic expression development, genomic imprinting, X-chromosome inactivation, and DNA repair. DNA methylation in humans occurs almost exclusively at the C5 position of CpG dinucleotides in genomic promoter regions. Alterations in DNA met...
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ژورنال
عنوان ژورنال: Clinical Chemistry
سال: 2008
ISSN: 0009-9147,1530-8561
DOI: 10.1373/clinchem.2007.098491